https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33427 Wed 31 Oct 2018 15:13:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54999 Wed 27 Mar 2024 16:39:08 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47720 Wed 25 Jan 2023 13:29:24 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47695 Wed 25 Jan 2023 08:49:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48605 Wed 22 Mar 2023 09:01:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29969 Phyllanthus amarus (P. amarus) is a herbal plant used in the treatment of various diseases such as hepatitis, diabetes, and cancer. Efficiency of its bioactive compounds extraction and therefore the biological activity of the extracts are significantly influenced by both solvent character and extraction method. This study is aimed at the determination of the influence of six various solvents (water, acetonitrile, ethanol, methanol, ethyl acetate, and dichloromethane) and nine different extraction methods (conventional, ultrasound-assisted, microwave-assisted, and six novel methods) on the extraction efficiency and antioxidant capacity of P. amarus. The results indicated that water extracted the maximal amount of phenolics from P. amarus and had the highest antioxidant capacity, while microwave-assisted extraction provided the highest yields of phenolics and saponins, and the highest antioxidant capacity with the lowest energy consumption when compared to the other extraction methods. These findings implied that water and microwave-assisted extraction are recommended as the most effective solvent and method for the extraction of bioactive compounds from P. amarus for potential application in the pharmaceutical and nutraceutical industries.]]> Wed 19 Jan 2022 15:19:25 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37096 Wed 17 Nov 2021 16:30:40 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32515 M. alba stem bark. Nephrotoxicity was induced with administration of paracetamol. Nephroprotection was studied using two doses of the extract. The experimental animals were divided into four groups (n = 6). Two groups served as positive and negative controls, respectively, and two received the test substances. All of the contents were orally administered. Significant reductions in nephrotoxicity and oxidative damages were observed in the treatment groups. There was a marked decrease in blood levels of urea, creatinine, and lipid peroxidation. Furthermore, it was found that glutathione levels in the blood increased dramatically after treatment. Histological findings confirmed the potent renoprotective potential of moralbosteroid. This was evidenced by the minimized intensity of nephritic cellular destruction. In animal studies, moralbosteroid exhibited dose-dependent activity, which is thought to be mediated through its antioxidant potential.]]> Wed 13 Jun 2018 11:02:23 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53640 Wed 13 Dec 2023 10:34:07 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26060 Wed 11 Apr 2018 16:22:58 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26324 Wed 11 Apr 2018 14:05:07 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19404 Wed 11 Apr 2018 13:19:57 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19242 Wed 11 Apr 2018 12:53:35 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26299 Wed 11 Apr 2018 11:50:48 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13954 Wed 11 Apr 2018 09:13:08 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53700 Wed 10 Jan 2024 10:48:50 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36554 Catharanthus roseus (L.) G. Don (C. roseus) stem. C. roseus stem was powdered and extracted with methanol using ultrasound-assisted extraction to obtain the crude extract. The crude extract was further fractioned using liquid-liquid extraction technique to obtain extracts of increasing polarity including n-butanol and residual aqueous fractions. The crude extract and its derived fractions were then subjected to phytochemical screening and assayed for antioxidant, antimicrobial and cytotoxic activities. Results showed that the n-butanol fraction contained the highest levels of saponins and phenolics (3037.54 mg ESE/g and 77.87 mg GAE/g, respectively) and possessed the strongest antioxidant capacity amongst the tested extracts. HPLC analysis revealed that this n-butanol fraction had high levels of apigenin and kaempferol, whereas the aqueous fraction contained a high level of gallic acid. The n-butanol fraction was found to effectively inhibit the activity of Escherichia coli and Staphylococccus lugdunensis. The n-butanol fraction also possessed strong cytotoxic activity in vitro against a wide range of cancer cell lines including A2780 (ovarian), H460 (lung), A431 (skin), MIA PaCa-2 (pancreas), Du145 (prostate), HT29 (colon), MCF-7 (breast), BE2-C (neuroblastoma), SJ-G2, U87 and SMA (glioblastoma) at low doses (GI50 values of 5.2−21.0 µg/mL). These results indicate that the n-butanol fraction prepared from C. roseus stem is a rich source of bioactive compounds which can be isolated for further evaluation as potential antimicrobial drugs or antitumor therapeutic agents.]]> Wed 09 Mar 2022 16:00:04 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24691 Phyllanthus amarus is known as a healing herb which has traditionally been used in the treatment of various diseases such as hepatitis, diabetes and cancer. The extraction parameters have great effects on the extraction efficiency of bioactive compounds and pharmacological activity of the extracts. This study sought to optimise the microwave-assisted extraction parameters for phenolic compounds-enriched extracts and antioxidant capacity from P. amarus using response surface methodology (RSM). The results showed that the optimal microwave-assisted extraction parameters were an extraction time of 30 min, an irradiation time of 14 s min⁻¹ and a ratio of solvent to sample of 150 mL g⁻¹. The total phenolic content, phenolic extraction efficiency, saponin content, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity, 2,2-diphenyl-1-picryl-hydrazil (DPPH) radical scavenging capacity and ferric reducing antioxidant power of the P. amarus achieved under these optimal parameters were 87.3 mg of gallic acid equivalents (GAE) per gram of dried sample, 69.7 %, 134.9 mg of escin equivalents (EE) per gram of dried sample, 997.8, 604.7 and 437.3 all in mg of trolox equivalents (TE) per gram of dried sample, respectively, which were not significantly different from the predicted values (86.9 mg of GAE per gram of dried sample, 67.3 %, 123.5 mg of EE per gram of dried sample, 1013.3 mg of TE per gram of dried sample, 530.6 mg of TE per gram of dried sample and 423.5 mg of TE per gram of dried sample, respectively). Accordingly, the optimal microwave-assisted extraction parameters of 30 min, 14 s min⁻¹ and 150 mL g⁻¹ are recommended for the extraction of enriched phenolics from P. amarus for potential application in the nutraceutical and pharmaceutical industries.]]> Wed 09 Feb 2022 15:54:22 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43950 Wed 05 Oct 2022 13:05:30 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43936 Wed 05 Oct 2022 12:44:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33080 −1, respectively. The carotenoid extraction yield and the antioxidant capacity extraction yield were 271 mg/100 g DW and 737 μm TE/100 g DW, respectively. Thus, the extraction using ethyl acetate with the ratio of 80:1 (mL solvent per g Gac peel) for 150 min at 40.7°C is suggested for recovering carotenoids and antioxidant capacity from Gac peel.]]> Wed 04 Sep 2019 09:54:30 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38104 Wed 04 Aug 2021 17:41:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36325 Tue 22 Jun 2021 13:00:04 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48526 Tue 21 Mar 2023 13:27:22 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43493 Tue 20 Sep 2022 15:35:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48949 Tue 18 Apr 2023 11:15:59 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26014 Tue 17 Mar 2020 11:33:29 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31507 Phyllanthus amarus (P. amarus) has been used as a medicinal plant for the prevention and treatment of chronic ailments such as diabetes, hepatitis, and cancer. Methods: The physicochemical properties, antioxidant and cytotoxic activities of crude extracts and fractions from P. amarus were determined using spectrophotometric method. Results: The P. amarus methanol (PAM) extract had lower levels of residual moisture (7.40%) and water activity (0.24) and higher contents of saponins, phenolics, flavonoids, and proanthocyanidins (1657.86 mg escin equivalents, 250.45 mg gallic acid equivalents, 274.73 mg rutin equivalents and 61.22 mg catechin equivalents per g dried extract, respectively) than those of the P. amarus water (PAW) extract. The antioxidant activity of PAM extract was significantly higher (p < 0.05) than that of the PAW extract, PAM fractions, and phyllanthin (known as a major compound in the P. amarus). Higher cytotoxic activity of PAM extract based on MTT assay on different cell lines including MiaPaCa-2 (pancreas), HT29 (colon), A2780 (ovarian), H460 (lung), A431 (skin), Du145 (prostate), BE2-C (neuroblastoma), MCF-7 (breast), MCF-10A (normal breast), and U87, SJ-G2, SMA (glioblastoma) was observed in comparison to the PAW extract and PAM fractions. The cytotoxic potential of the PAW extract (200 µg/mL), based on the CCK-8 assay on a pancreatic cancer cell line (MiaCaPa2) was significantly lower (p < 0.05) than those of gemcitabine (50 nM) and a saponin-enriched extract from quillajia bark at 200 µg/mL (a commercial product), but was significantly higher than that of phyllanthin at 2 µg/mL. Conclusions: The results achieved from this study reveal that the PA extracts are a potential source for the development of natural antioxidant products and/or novel anticancer drugs.]]> Tue 17 Mar 2020 11:32:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54788 Tue 12 Mar 2024 14:34:36 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44237 Artemisia vulgaris is a traditional Chinese herb believed to have a wide range of healing properties; it is traditionally used to treat numerous health ailments. The plant is commonly called mugwort or riverside wormwood. The plant is edible, and in addition to its medicinal properties, it is also used as a culinary herb in Asian cooking in the form of a vegetable or in soup. The plant has garnered the attention of researchers in the past few decades, and several research studies have investigated its biological effects, including antioxidant, anti-inflammatory, anticancer, hypolipidemic, and antimicrobial properties. In this review, various studies on these biological effects are discussed along with the tests conducted, compounds involved, and proposed mechanisms of action. This review will be of interest to the researchers working in the field of herbal medicine, pharmacology, medical sciences, and immunology.]]> Tue 11 Oct 2022 12:13:02 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33074 Momordica cochinchinensis Spreng.) is a rich source of carotenoids for the manufacture of powder, oil and capsules for food, cosmetic and pharmaceutical uses. Currently, only the aril of the Gac fruit is processed and the peel, similar to the other components, is discarded, although it contains high level of carotenoids, which could be extracted for commercial use. In the present study, four different drying methods (hot‐air, vacuum, heat pump and freeze drying), different temperatures and drying times were investigated for producing dried Gac peel suitable for carotenoid extraction. Results: The drying methods and drying temperatures significantly affected the drying time, carotenoid content and antioxidant capacity of the dried Gac peel. Among the investigated drying methods, hot‐air drying at 80°C and vacuum drying at 50°C produced dried Gac peel that exhibited the highest retention of carotenoids and the strongest antioxidant capacity. Conclusion: Hot‐air drying at 80°C and vacuum drying at 50°C are recommended for the drying of Gac peel.]]> Tue 03 Sep 2019 18:30:56 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34442 Tue 03 Sep 2019 18:00:55 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33078 Momordica cochinchinensis Spreng.) contains high levels of bioactive compounds, especially carotenoids which possess significant antioxidant capacities. However, the peel of Gac is regarded as a waste from the production of carotenoid‐rich oil from Gac fruit. In this study, carotenoids of Gac peel were extracted by microwave‐assisted extraction (MAE) and ultrasound‐assisted extraction (UAE) using ethyl acetate as extraction solvent. The effect of extraction time and different levels of microwave and ultrasonic powers on the yield of total carotenoid and antioxidant capacity of the extracts were investigated. The results showed that an extraction at 120 W for 25 min and an extraction at 200 W for 80 min were the most effective for MAE and UAE of the Gac peel samples, respectively. The maximum carotenoid and antioxidant capacity yields of UAE were significantly higher than those of the MAE. The antioxidant capacity of extract obtained by the UAE was also significantly higher that of the conventional extraction using the same ratio of solvent to material. The results showed that both MAE and UAE could be used to reduce the extraction time significantly in comparison with conventional extraction of Gac peel while still obtained good extraction efficiencies. Thus, MAE and UAE are recommended for the improvement of carotenoid and antioxidant capacity extraction from Gac peel.]]> Tue 03 Sep 2019 17:53:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50551 Tue 01 Aug 2023 10:53:37 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42563 Thu 25 Aug 2022 11:12:18 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33062 Paramignya trimera (Oliv.) Guillaum) has been used as a medicinal plant for cancer prevention and treatment in recent years. The objective of this study was to determine the physicochemical, antioxidant, and cytotoxic properties of crude P. trimera root (PTR) extract and its fractions using MeOH as a solvent and microwave‐assisted extraction as an advanced technique for preparation of the PTR extract. The results showed that the PTR extract had high contents of saponins, phenolics, flavonoids, and proanthocyanidins (7731.05 mg escin equiv. (EE), 238.13 mg gallic acid equiv. (GAE), 81.49 mg rutin equiv., and 58.08 mg catechin equiv. (CE)/g dried extract, resp.). Antioxidant activity of PTR extract was significantly higher (P < 0.05) than those of four its fractions and ostruthin, a key bioactive compound in the P. trimera, while potent cytotoxic capacity of PTR extract on various cancer cell lines in terms of MiaPaCa‐2 (pancreas), HT29 (colon), A2780 (ovarian), H460 (lung), A431 (skin), Du145 (prostate), BE2‐C (neuroblastoma), MCF‐7 (breast), MCF‐10A (normal breast), and U87, SJ‐G2, SMA (glioblastoma) was observed with GI₅₀ values ranging from 15 to 32 μg/ml. Cytotoxic potential on pancreatic cancer cells of PTR extract (100 – 200 μg/ml) was significantly higher (P < 0.05) than those of its four fractions (50 μg/ml), ostruthin (20 μg/ml) and gemcitabine (50 nm), and being comparable to a saponin‐enriched extract from quillajia bark, a commercial product. Based on the results achieved, we can conclude that the PTR extract is a potential source for application of in the nutraceutical, medical, and pharmaceutical industries.]]> Thu 17 Feb 2022 09:31:57 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36553 Catharanthus roseus (L.) G. Don (C. roseus) is a well-known medicinal plant for its source of alkaloids solely found in the leaves. Other parts including the root are usually discarded after the alkaloid extraction. This study sought to investigate phytochemical profiles, antioxidant, antimicrobial and cytotoxic properties of the C. roseus root extract (RE) and its two sub-fractions including saponin-enriched (SE) and aqueous (AQ) fractions. The results showed that the RE was a rich source of saponins (1744.44 mg ESE/g) and phenolics (51.27 mg GAE/g), which comprised of gallic acid (25.74 mg/g), apigenin (1.45 mg/g) and kaempferol (1.58 mg/g). The SE fraction was enriched with 31% of saponins and 63% of phenolics higher than those of the RE; whereas the concentrations of saponins and phenolics of the AQ fraction were lower than those of the RE by 40% and 74%, respectively. The content of gallic acid in the SE fraction was 1.4-fold and 1.5-fold higher than those of the RE or AQ fraction, respectively. The SE fraction demonstrated potent antioxidant capacity, which was significantly higher than the RE or AQ fraction, and also exhibited strong anti-proliferative activity against 11 cancer cell lines including A2780 (ovarian), H460 (lung), A431 (skin), MIA PaCa-2 (pancreas), Du145 (prostate), HT29 (colon), MCF-7 (breast), BE2-C (neuroblastoma), SJ-G2, U87 and SMA (glioblastoma) with low GI₅₀ values (≤ 2.00 µg/mL). The SE fraction was also shown to effectively inhibit the growth of both bacteria (Escherichia coli, Enterobacter aerogenes and Staphylococccus lugdunensis) and fungi (Candida albicans and Aspergillus niger). These findings warrant further investigation to isolate major compounds from the SE fraction and further test their antioxidant, anticancer and antimicrobial activities.]]> Thu 17 Feb 2022 09:27:14 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23950 Paramignya trimera (Oliv.) Guillaum) has been used as an herbal medicine for the treatment of cancer or cancer-like diseases in recent years, particularly in Vietnam. Drying is an important step for preparation of dried materials for storage and further investigation; however, the effects of drying must be taken into account when processing samples, because this can have profound effects on the stability of phytochemical compounds and the biological activity of the dried P. trimera root. As such, this study assessed the effects of four different drying methods (conventional, hot air, vacuum, and microwave) on phytochemical retention and antioxidant capacity of P. trimera root, to identify an optimal drying method for P. trimera root. The results showed that the drying methods significantly affected phytochemical levels and antioxidant capacity of P. trimera root and that among the four drying methods tested, microwave drying (400 W) had the highest levels of phytochemical compounds, with total phenolic, total flavonoid, proanthocyanidin, and saponin contents of 11.27 mg GAE, 19.88 mg RE, 3.98 mg CE, and 267.15 mg EE/gram of dried sample, respectively. Dried sample prepared using this method had antioxidant capacity comparable to that of other drying methods. In addition, this method had the shortest drying time (0.28 h) and consumed the least energy (0.28 kWh). Therefore, microwave drying should be considered for drying P. trimera root for further investigation and utilization.]]> Thu 17 Feb 2022 09:26:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50836 Thu 17 Aug 2023 11:57:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53748 Thu 11 Jan 2024 12:15:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40628 Thu 11 Aug 2022 11:14:14 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46060 0.05). Thus, soaking is not required for the ETHOS X extraction of polyphenols from LSTT leaves. RSM was successfully applied to explore the impact of ETHOS X extraction conditions and optimize the extraction conditions. Radiation time significantly affects the recovery yield of phenolics (p < 0.05) positively, whereas irradiation power and sample to solvent ratio adversely influenced the extraction yields of phenolics. The optimal ETHOS X extraction conditions were: radiation time of 60 min, irradiation power of 600 W, and sample to solvent ratio of 2 g/100 mL. Under these conditions, 119.21 ± 7.09 mg of phenolic, 85.31 ± 4.55 mg of flavonoids, and 137.51 ± 12.52 mg of proanthocyanidins can be extracted from a gram of dried LSTT leaves. In comparison with UAE and SWB, ETHOS X is not more effective for the extraction of phenolics than UAE and SWB. However, this technique can save half of the solvent volume compared to UAE and SWB techniques.]]> Thu 10 Nov 2022 11:28:04 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46936 Thu 08 Dec 2022 11:38:45 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29794 Thu 04 Nov 2021 10:39:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46816 Thu 01 Dec 2022 09:46:26 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31233 Sat 24 Mar 2018 08:43:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:897 Sat 24 Mar 2018 08:29:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11846 Sat 24 Mar 2018 08:09:52 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10730 Sat 24 Mar 2018 08:09:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20454 Sat 24 Mar 2018 08:06:57 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17836 Sat 24 Mar 2018 08:03:31 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20744 2t concentration were assessed. The experimental procedure was repeated with the remaining supplement after a 3-wk washout. Eight participants completed both supplementation trials. Results: NAC improved sprint performance during the cycle ergometer race simulation (P < 0.001, η_p² = 0.03). Supplementation with NAC also augmented postexercise plasma total antioxidant capacity (P = 0.005, η_p² = 0.19), reduced exercise-induced oxidative damage (plasma thiobarbituric acid-reactive substances, P = 0.002, η_p² = 0.22; urinary 15-isoprostane F_2t concentration, P = 0.010, η_p² = 0.431), attenuated inflammation (plasma interleukin 6, P = 0.002, η_p² = 0.22; monocyte chemotactic protein 1, P = 0.012, η_p² = 0.17), and increased postexercise nuclear factor κB activity (P < 0.001, η_p² = 0.21). Conclusion: Oral NAC supplementation improved cycling performance via an improved redox balance and promoted adaptive processes in well-trained athletes undergoing strenuous physical training.]]> Sat 24 Mar 2018 08:00:24 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20089 Sat 24 Mar 2018 08:00:09 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20302 Sat 24 Mar 2018 07:55:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20130 Sat 24 Mar 2018 07:51:33 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21347 Sat 24 Mar 2018 07:51:29 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18779 Sat 24 Mar 2018 07:51:09 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6652 Sat 24 Mar 2018 07:46:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29651 Sat 24 Mar 2018 07:41:56 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28484 Davidsonia pruriens F. Muell) and to assess the physicochemical and antioxidant properties of the phenolic-enriched extract. The results showed that temperature, time and ratio significantly affected the extraction of TPC. Optimization of extraction conditions was performed using response surface methodology (RSM) utilising a Box–Behnken design. Optimal extraction conditions were determined to be temperature: 90 °C, extraction time: 30 min and solvent to mass ratio: 20:1 mL g⁻¹. The extracted solid obtained under these conditions had low-moisture content, high water solubility and contained 45 mg GAE g−1 of TPC, 22 mg RUE g⁻¹ of flavonoids, 3.2 mg CAE g⁻¹ of proanthocyanidins, 2 mg CGE g⁻¹ of anthocyanidins and 56 mg ACE g⁻¹ vitamin C. The extract possessed potent antioxidant capacity, but was comparatively lower than those of vitamin E and BHT. Thus, Davidson's plum should be further investigated for its potential health promoting benefits and utilisation in the nutraceutical and food industries.]]> Sat 24 Mar 2018 07:39:36 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27329 Eucalyptus robusta (Sm.) (ER) is a widely distributed tree native to the east coast of Australia, which has also been established in numerous other countries. ER leaves contain high levels of essential oils and are rich in total phenolic compounds (TPC), which have been linked with health benefits; however, there is limited information on the bioactivity of ER leaf extracts. This study aimed to optimise water extraction conditions for TPC, prepare a spray-dried powdered extract and test its physicochemical, antioxidant and anti-proliferative properties. The results showed that optimal water extraction conditions for TPC were 85°C, 15 min and a water-to-leaf ratio of 20:1 mL/g. Under these conditions, spray-dried powdered extract was prepared with a recovery yield of 85%. The extract was water-soluble and had a TPC level of 407 mg GAE/g. It also possessed potent antioxidant capacity, comparable to pure ascorbic acid, but higher than pure α-tocopherol. In addition, the powdered extract demonstrated significant activity against a panel of cancer cell lines, which included cancers of the pancreas, breast, lung, brain, skin, colon and ovary. Of note, the ER extract exerted a more significant toxic effect on pancreatic cancer (PC) cells compared to gemcitabine, the first line chemotherapeutic agent for PC. We suggest that future studies should purify individual bioactive compounds from ER for further investigation of its potential health promoting and anti-cancer activity.]]> Sat 24 Mar 2018 07:38:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27632 Sat 24 Mar 2018 07:34:05 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28857 Sat 24 Mar 2018 07:33:17 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25688 Sat 24 Mar 2018 07:28:23 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23861 Sat 24 Mar 2018 07:12:11 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49707 Mon 29 May 2023 13:00:57 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43619 Mon 26 Sep 2022 16:08:45 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40415 Cupaniopsis anacardioides) is an Australian native tree, possessing high level bioactivity and antioxidant activity. To prevent deterioration of active constituents, appropriate drying practices must be determined. This study comparatively evaluates the impact of a range of drying methods including freeze-, microwave-, vacuum-, hot air- and sun-drying on the physical, phytochemical and antioxidant characteristics of Tuckeroo fruit. Experimental results showed that the five drying methods had significant impact on the physicochemical properties and antioxidant activity of the fruits. Of the drying methods assessed, freeze drying best preserved Tuckeroo activity, recording higher total phenolic content (TPC) (81.88 mg gallic acid equivalent (GAE)/g), total flavonoids (TFC) (107.71 mg catechin equivalent (CAE)/g), proanthocyanidins (TPro) (83.86 mg CAE/g) and exhibited the strongest antioxidant capacity. However, vacuum drying at 65 kPa, 100 ℃ for 5 h is recommended for drying Tuckeroo fruits for further processing in a large scale as it also retained high levels of TPC, TFC and TPro (58 mg GAE/g, 91 mg CAE/g and 74 mg CAE/g, respectively).]]> Mon 11 Jul 2022 14:44:41 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45802  94 cm in males, > 80 cm in females) or inflammation (hs-CRP > 1 mg/L). In the subgroup with abdominal obesity, ox-LDL decreased by 13.5 mU/mL (95% CI − 23.5 to − 3.6) and TAC increased by 0.04 mM (95% CI 0.006–0.07) only after HPOO consumption. In the subgroup with inflammation, hs-CRP decreased by 1.9 mg/L (95% CI − 3.7 to −0.1) only in the HPOO arm. Conclusions: Although there were no significant differences between treatments, the changes observed after HPOO consumption demonstrate the antioxidant and anti-inflammatory effect of this oil, which is more pronounced in adults with high cardiometabolic risk (Clinical Trial Registration: ACTRN12618000706279).]]> Mon 07 Nov 2022 10:05:13 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33748 Mon 07 Jan 2019 10:45:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36529 Fri 29 May 2020 13:47:49 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33079 Fri 24 Aug 2018 15:01:54 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33060 Phyllanthus amarus. The findings indicated that the optimal MAE parameters consisted of 100% methanol, irradiation time 4 s/min, extraction time 50 min, and solvent to sample ratio 50 mL/g. Under these optimal parameters, saponin content (SC), saponin extraction efficiency (SEE), and total phenolic content (TPC) of P. amarus were 229.5 mg escin equivalents (EEs)/g dried sample, 82.8%, and 40.7 mg gallic acid equivalents (GAEs)/g dried sample, respectively. The antioxidant capacity of P. amarus in terms of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging capacity (ARSC), 2,2-diphenyl-1-picryl-hydrazil radical scavenging capacity (DRSC), and ferric reducing antioxidant power (FRAP) were 487.3, 330.6, and 233.5 mg trolox equivalents (TEs)/g dried sample, respectively. These measured values were not significantly different from the predicted values by response surface methodology (227.9 mg EE/g dried sample, 82.1%, and 39.2 mg GAE/g dried sample for SC, SEE, and TPC and 484.8, 297.3, and 226.6 mg TE/g dried sample for ARSC, DRSC, and FRAP, respectively). Hence, the optimal MAE parameters are suggested for effective extraction of saponins from P. amarus for further investigations and applications.]]> Fri 24 Aug 2018 12:55:58 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54944 Fri 22 Mar 2024 15:20:51 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34477 Musa cavendish) peel using response surface methodology. The results showed that recovery yields of phenolic compounds, flavonoids, proanthocyanidins and antioxidant properties were affected by the extraction parameters; of which the acetone concentration had the greatest effect. Optimal extraction conditions were found to be at ultrasonic temperature of 30°C, ultrasonic time of 5 min, ultrasonic power of 150 W, sample to solvent ratio of 8:100 g/mL and acetone concentration of 60%. Under these optimal conditions, 23.49 mg of phenolic compounds, 39.46 mg of flavonoids and 13.11 mg of proanthocyanidins could be extracted from 1 g of banana (M. cavendish) peel. Practical applications: Banana peel known as waste is generated in a big quantity with limited utilization. Therefore, it is necessary to utilize this by‐product for adding value to food industry. This study was designed to establish a simple, effective extraction method for maximum recovery of phenolic compounds from banana peel. Findings from this study can be used for further isolation and purification of phenolic compounds from banana peel for subsequent application in nutraceutical and pharmaceutical industry.]]> Fri 15 Mar 2019 13:30:50 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41789 Fri 12 Aug 2022 12:17:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32830 Fri 03 Dec 2021 10:34:06 AEDT ]]>